Journal: Biomedical Optics Express

Article Title: Large-scale, cell-resolution volumetric mapping allows layer-specific investigation of human brain cytoarchitecture

doi: 10.1364/BOE.415555

Figure Lengend Snippet: Table summarizing the dyes tested in this study. The P/M column denotes polyclonal vs monoclonal antibodies. The same abbreviations used in are used. AF is a shorthand for Alexa Fluor.

Article Snippet: Sample pictures before and after the clearing process were acquired using a digital camera (Sony DSC-WX500), samples were kept soaked either in PBS or TDE. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Molecule Company Cat. n. Host P/M Dilution NeuN Merck ABN91 Chicken P 1:50 GAD67 Santa Cruz sc-28376 Mouse M 1:200 GAD65/67 St John’s Lab STJ93195 Rabbit P 1:200 PV Abcam ab11427 Rabbit P 1:200 PV Abcam ab32895 Goat P 1:200 CB Abcam ab207528 Rabbit M 1:200 VIP Abcam ab214244 Rabbit M 1:200 SST Abcam ab30788 Rat M 1:200 NPY Abcam ab6173 Sheep P 1:200 NPY Abcam ab112473 Mouse M 1:200 SMI-32 Merck NE1023 Mouse M 1:200 Neurofilament Abcam ab4680 Chicken P 1:200 GluS Merck MAB302 Mouse M 1:200 MAP2 Abcam ab5392 Chicken P 1:200 GFAP Abcam ab194324 Rabbit M 1:200 Iba1 Abcam ab195031 Rabbit M 1:200 Coll IV Abcam ab6586 Rabbit P 1:200 Vim Abcam ab8069 Mouse M 1:200 Anti-Rat IgG, AF 568 Abcam ab175475 Donkey P 1:200 Anti-Rabbit IgG, AF 568 Abcam ab175470 Donkey P 1:200 Anti-Chicken IgY, AF 568 Abcam ab175711 Goat P 1:200 Anti-Mouse IgG, AF 568 Abcam ab175700 Donkey P 1:200 Anti-Sheep IgG, AF 568 Abcam ab175712 Donkey P 1:200 Anti-Rabbit IgG, AF 488 Abcam ab150077 Goat P 1:200 Anti-Chicken IgY, AF 488 Abcam ab150169 Goat P 1:200 DAPI Thermo Fisher Scientific D1306 1:1000 SYTOX Green Thermo Fisher Scientific S7020 1:1000 Open in a separate window caption a8 Table summarizing the dyes tested in this study.

Techniques: Bioprocessing

Journal: eNeuro

Article Title: Numbers of Granule Cells and GABAergic Boutons Are Correlated in Shrunken Sclerotic Hippocampi of Sea Lions with Temporal Lobe Epilepsy

doi: 10.1523/ENEURO.0389-25.2026

Figure Lengend Snippet: Adult female control sea lion hippocampus. Nissl stain ( A ), glutamic acid decarboxylase (GAD)67-immunoreactivity to label cell bodies ( B ), and vesicular γ-amino butyric acid vesicular transporter (VGAT) plus GAD65/67-immunoreactivity to label synaptic boutons ( C ).

Article Snippet: Starting at a random point near the septal pole, a 1-in-24 series of sections was sampled and processed with a staining protocol optimized to label GABAergic synaptic boutons in rat tissue , using the same antibodies as for sea lions: one to GAD65/67 (1:60,000, L127/12, UC Davis/NIH NeuroMab Facility) and the other to VGAT (1:3,000, MSFR106160, Frontier Institute), but preceded by antigen retrieval in 0.5 M citrate buffer with 1% NaCl (pH 8.6) at 90°C for 70 min and avidin/biotin blocking.

Techniques: Control, Staining

Journal: eNeuro

Article Title: Numbers of Granule Cells and GABAergic Boutons Are Correlated in Shrunken Sclerotic Hippocampi of Sea Lions with Temporal Lobe Epilepsy

doi: 10.1523/ENEURO.0389-25.2026

Figure Lengend Snippet: Dentate gyrus of the non-sclerotic ( A–C ) and sclerotic ( D–F ) hippocampi of a subadult female sea lion. Nissl staining ( A , D ) reveals neuron loss in the hilus (H) and granule cell layer (G) of the sclerotic hippocampus. Immunolabeling for γ-amino butyric acid vesicular transporter (VGAT) plus GAD65/67 ( B , C , E , F ) reveals GABAergic synaptic boutons. M, molecular layer; I, inner molecular layer.

Article Snippet: Starting at a random point near the septal pole, a 1-in-24 series of sections was sampled and processed with a staining protocol optimized to label GABAergic synaptic boutons in rat tissue , using the same antibodies as for sea lions: one to GAD65/67 (1:60,000, L127/12, UC Davis/NIH NeuroMab Facility) and the other to VGAT (1:3,000, MSFR106160, Frontier Institute), but preceded by antigen retrieval in 0.5 M citrate buffer with 1% NaCl (pH 8.6) at 90°C for 70 min and avidin/biotin blocking.

Techniques: Staining, Immunolabeling

Journal: Transgenic Research

Article Title: Comparative analysis of different biofactories for the production of a major diabetes autoantigen

doi: 10.1007/s11248-013-9749-9

Figure Lengend Snippet: Transient expression of hGAD65 and hGAD65mut in Nicotiana benthamiana plants using vector pK7WG2. Left panel western blot of hGAD65 (G65) and hGAD65mut (G65m) in leaf extracts (5 μg TSP per lane) detected using the GC3108 antibody. Right panel loading control stained with Coomassie Brilliant Blue. Numbers indicate the molecular mass markers in kDa. n.c. negative control, leaves infiltrated with the pK7WG2 vector carrying the gfp marker gene; p.c. positive control, 10 ng of commercial rhGAD65-His 6 produced in the baculovirus/insect cell system

Article Snippet: Proteins were detected using the GC3108 (IgG1) monoclonal antibody (Biomol International, Farmingdale, NY) as previously described (Avesani et al. ).

Techniques: Expressing, Plasmid Preparation, Western Blot, Control, Staining, Negative Control, Marker, Positive Control, Produced

Journal: Transgenic Research

Article Title: Comparative analysis of different biofactories for the production of a major diabetes autoantigen

doi: 10.1007/s11248-013-9749-9

Figure Lengend Snippet: Transient expression of hGAD65 and hGAD65mut in Nicotiana benthamiana plants using MagnICON vectors. Left panel western blot of hGAD65 (G65) and hGAD65mut (G65m) in leaf extracts (1 μg TSP per lane) detected using the GC3108 antibody. Right panel loading control stained with Coomassie Brilliant Blue. Numbers indicate the molecular mass markers in kDa. n.c. negative control, plant infiltrated with the pICH20111 5′-module and pICH14011 integrase-module; p.c. positive control, 10 ng of commercial rhGAD65-His 6 produced in the baculovirus/insect cell system

Article Snippet: Proteins were detected using the GC3108 (IgG1) monoclonal antibody (Biomol International, Farmingdale, NY) as previously described (Avesani et al. ).

Techniques: Expressing, Western Blot, Control, Staining, Negative Control, Positive Control, Produced

Journal: Transgenic Research

Article Title: Comparative analysis of different biofactories for the production of a major diabetes autoantigen

doi: 10.1007/s11248-013-9749-9

Figure Lengend Snippet: Expression of hGAD65 and hGAD65mut using the baculovirus/insect cell platform. Left panel western blot of hGAD65 (G65) and hGAD65mut (G65m) in cell extracts (15 μg TSP per lane) detected using the GC3108 antibody. Right panel loading control stained with Coomassie Brilliant Blue. Numbers indicate the molecular mass markers in kDa. n.c. negative control, non-transformed insect cell extracts; p.c. positive control, 10 ng of commercial rhGAD65-His 6 produced in the baculovirus/insect cell system

Article Snippet: Proteins were detected using the GC3108 (IgG1) monoclonal antibody (Biomol International, Farmingdale, NY) as previously described (Avesani et al. ).

Techniques: Expressing, Western Blot, Control, Staining, Negative Control, Transformation Assay, Positive Control, Produced

Journal: Transgenic Research

Article Title: Comparative analysis of different biofactories for the production of a major diabetes autoantigen

doi: 10.1007/s11248-013-9749-9

Figure Lengend Snippet: Expression of hGAD65 and hGAD65mut using the E. coli inducible expression platform. Left panel western blot of hGAD65 (G65) and hGAD65mut (G65m) in cell extracts (2 μg TSP per lane) detected using the GC3108 antibody. Right panel loading control stained with Coomassie Brilliant Blue, with asterisk indicating the band corresponding to hGAD65mut. Numbers indicate the molecular mass markers in kDa. n.c. negative control, bacterial cell transformed with the pDest17 ‘empty’ vector; p.c. positive control, 15 ng of commercial rhGAD65-His 6 produced in the baculovirus/insect cell system

Article Snippet: Proteins were detected using the GC3108 (IgG1) monoclonal antibody (Biomol International, Farmingdale, NY) as previously described (Avesani et al. ).

Techniques: Expressing, Western Blot, Control, Staining, Negative Control, Transformation Assay, Plasmid Preparation, Positive Control, Produced

Journal: Nature Communications

Article Title: Functional neuronal circuitry and oscillatory dynamics in human brain organoids

doi: 10.1038/s41467-022-32115-4

Figure Lengend Snippet: a Top, high-resolution, whole-section of an 8-month organoid immunostained with anti-GFAP (green), and counterstained with Hoechst (cell nuclei, blue), scale bar 1 mm. Middle, a high magnification of (top box) showing stellate appearance characteristic of astrocytes. Bottom left, Anti-GFAP-positive astrocytes (green) in an 8-month organoid co-labeled with anti-connexin 43 demonstrating gap junctions (red). Bottom right, connexin 43 gap junction (red; arrows). Middle and bottom scale bars are 40 µm. b Top, long neuronal processes labeled with anti-SMI312 (green) in an 8-month organoid, scale bar 500 µm. Bottom, anti-SMI312 (green) axons neighboring MAP2-positive (red) neurons near the margin of 8-month organoid, scale bar 20 µm. c Top, anti-GAD65 positive neurons (green) co-labeled with anti-MAP2. Bottom, Anti-Parvalbumin-positive neurons (green) in an 8-month organoid co-labeled with anti-MAP2 (red). Scale bars 40 µm. d The presynaptic marker synaptobrevin label MAP2-positive processes as puncta, scale bar 20 µm. Data from a – d were repeated independently on n = 3 organoids. e Single-cell RNA sequencing (drop-seq) shows the presence of glutamatergic neurons, GABAergic neurons and astrocyte populations. Single-cell transcriptomes (5680 cells collected from three 7-month-old organoids) are visualized as a Uniform Manifold Approximation and Projection (UMAP). Source data are provided as a Source Data File.

Article Snippet: Primary antibodies used were anti-Parvalbumin (1:200; abcam, Cambridge, MA; ab11427), anti-GAD65 (1:200; GeneTex, Irvine, CA; GTX113192), anti-SMI312 (1:500; BioLegend, San Diego, CA; 801701), anti-GFAP (1:500; abcam, Cambridge, MA; ab53554), anti-connexin 43 (1:200; Millipore, Burlington, MA; MAB3067), anti-MAP2 (1:500; GeneTex, Irvine, CA; GTX82661), anti-synaptobrevin (1:500; Synaptic Systems, Goettingen, Germany; 104-211).

Techniques: Labeling, Marker, RNA Sequencing Assay

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Correlation analysis between cytokines’ profile, autoimmune antibodies and the duration of type 1 diabetes: A case control study in a specialized children’s centre in Riyadh

doi: 10.1177/03946320231209821

Figure Lengend Snippet: Summarizing the main results for the current research article allied with the previous knowledge related to the role of cytokines among T1D patients. (a) Normal mechanisms for the normal secretion of the insulin from the β-cells. (b) Theoretical initiation of T cells attacks and destroying the β-cells and reduces the amount of secreted insulin due to the recognition of auto-reactive T cells to the self-antigen present over MHC and destroying the β-cells. IFNγ cytokine plays an important role in activating natural killer (NK) cells, macrophages and CD8+ T cells (cytotoxic T cells) for destroying the β-cells. (c) Development of humoral immune response by activating the B cells to release autoantibodies against (GAD and islet); those autoantibodies can positively have correlated with the duration of T1D pathogenesis.

Article Snippet: Anti-human glutamic acid decarboxylase autoantibodies (GAD-65 Ab) were determined using the ELISA kit from (Biotang™, HU9498) with a minimum detection limit of 0.3 U/mL.

Techniques:

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Correlation analysis between cytokines’ profile, autoimmune antibodies and the duration of type 1 diabetes: A case control study in a specialized children’s centre in Riyadh

doi: 10.1177/03946320231209821

Figure Lengend Snippet: Linear regression analysis of the duration of diabetes in association with autoimmune markers and C-peptide level adjusted with levels circulating cytokines’, age, sex, and BMI.

Article Snippet: Anti-human glutamic acid decarboxylase autoantibodies (GAD-65 Ab) were determined using the ELISA kit from (Biotang™, HU9498) with a minimum detection limit of 0.3 U/mL.

Techniques: